1. Synthesis and characterization of phenothiazine based dye complexes for photodynamic therapy. 2. Synthesis and characterization of lithium ion fluorophores. Prakash B Sanjeevaiah

ISBN: 9780549944386

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NOOKstudy eTextbook

193 pages


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1. Synthesis and characterization of phenothiazine based dye complexes for photodynamic therapy. 2. Synthesis and characterization of lithium ion fluorophores.  by  Prakash B Sanjeevaiah

1. Synthesis and characterization of phenothiazine based dye complexes for photodynamic therapy. 2. Synthesis and characterization of lithium ion fluorophores. by Prakash B Sanjeevaiah
| NOOKstudy eTextbook | PDF, EPUB, FB2, DjVu, talking book, mp3, RTF | 193 pages | ISBN: 9780549944386 | 9.66 Mb

1. Synthesis and characterization of phenothiazine based dye complexes for photodynamic therapy. Photodynamic therapy (PDT) is an innovative approach for the treatment of cancer utilizing photosensitizers, light and oxygen. Phenothiazine-basedMore1. Synthesis and characterization of phenothiazine based dye complexes for photodynamic therapy. Photodynamic therapy (PDT) is an innovative approach for the treatment of cancer utilizing photosensitizers, light and oxygen. Phenothiazine-based photosensitizers are effective for PDT because of their high quantum efficiency, absorbance of light in the visible region of the electromagnetic spectrum, ease of modification, biodegradability among other properties make them ideal candidates.

Previous studies in our labs have used dyes such as Azure B, Malachite green and Fluorescein. These dye molecules were chemically modified and bound to hemoglobin and subsequent quantum efficiency and oxygen binding studies were carried out. Purity of the starting materials and finding ways to effectively purify and characterize the modified Dye-Hemoglobin complex posed challenges to previous workers. Addressing the purity issues as well as developing an efficient method for synthesizing Dye-Folate complexes with folic acid attached to guide the complex to a specific tumor target formed the basis of the current research.-Phenothiazine was used as the starting material and several modifications have been prepared in order to effectively bind with hemoglobin and folic acid.

The purity issues as well as the reactivity have been addressed. The synthesized complexes, including a Malachite green dye-folate complex, were characterized and subjected to spectroanalytical studies. The most promising candidates were then tested on tumor cell lines.-2. Synthesis and characterization of Li+ fluorophores.

Lithium salts continue to be the most promising drugs in the treatment of acute episodes and the prevention of relapses in patients suffering from bipolar disorder (also known as manic depression). The pharmacologic mode of action of Li+ is still a matter of conjecture and a detailed understanding of the biological action of Li+ is lacking. The therapeutic range of Li+ (0.5 to 1.2 mM) is not far from its toxic range (>2.0 mM). Previous studies have focused on Li+ interactions in biological systems using 7Li Nuclear magnetic resonance (NMR) and 7Li Magnetic resonance imaging (MRI) techniques which are not sensitive at millimolar levels.

These reasons necessitate the development of a Li+ selective fluorescent dye that can be used to measure Li+ concentrations in the therapeutic range. Previous attempts at the development of Li+ sensitive fluorescent dyes had limited success, because the indicators did not provide sufficiently large Li+/Na+ selectivity ratios.-It is well established that alkali metal ions, such as Li+, bind selectively to crown ethers based on an appropriate match between the cavity sizes of crown ethers and the ionic diameters of alkali metals.

7Li NMR data has shown that Li+ binds selectively to the cavity moiety of 12C4 crown ethers which was also suggested by previous work on Na+ fluorescent indicators based on the 18C6 and 15C5 Na+ selective functionalities.-In our current research, we have developed and characterized some 12C4 crown ether based fluorescent dyes.

The indicators were tested for fluorescent properties and were then subjected to in vitro specificity studies using human cell lines.



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